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2 edition of Thiobacillus ferrooxidans and soluble exopolymer production found in the catalog.

Thiobacillus ferrooxidans and soluble exopolymer production

Avik Nath

Thiobacillus ferrooxidans and soluble exopolymer production

by Avik Nath

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  • 30 Currently reading

Published by Laurentian University, Department of Biology in Sudbury, Ont .
Written in English


Edition Notes

StatementAvik Nath
The Physical Object
Paginationvii, 41 l. :
Number of Pages41
ID Numbers
Open LibraryOL20720117M

Kurosawa H, Maeda K, Yamamito E, Nakamura K, Amano Y. Growth of Thiobacillus ferrooxidans utilized thiosulfate through the oxidation route with iron recycling. J General Appl Microb – Google Scholar. Lacey DT, Lawson F. Kinetics of liquid phase oxidation of acid FeSO 4 by the bacterium Thiobacillus ferrooxidans. Heavy metals removal by bioleaching using Thiobacillus ferrooxidans Romanian Biotechnological Letters, Vol. 23, No. 2, In conclusion all studied metals were leached very well during the 12 weeks of extraction, the most effective extraction being performed during seventh and eighth week.

A positive correlation between numbers of T. ferrooxidans and acid production in coal refuse in the laboratory was demonstrated with the FA technique. Full text Get a printable copy (PDF file) of the complete article (M), or click on a page image below to browse page by page. medium, T. ferrooxidans is remarkably resistant to high concentrations ofmetalions exceptmercuricandsilver ions (, 13, 16, 17, 20). In contrast, whengrownonsulfur-salts medium, the organism is sensitive to those heavy metals. Tuovinen et al. reported that cobalt was the most toxic cation on the growth of T. ferrooxidans on sulfur-salts.

ofT. ferrooxidans. Myerson and Kline (17) and Dispitero et al. (8) reported that T. ferrooxidans couldadhereto coal andpyrite in afew minutes. Bagdigian and Myerson (3) suggested that the number of T. ferrooxidans cells that adhered to coal in-creasedwith an increase in the pyritic sulfur content ofthe coal. Similarobservations. Other articles where Acidithiobacillus ferrooxidans is discussed: bacteria: pH: sulfide deposits are attacked by A. ferrooxidans to generate sulfuric acid, which lowers the pH to or even However, acid tolerance of A. ferrooxidans applies only to sulfuric acid, since these bacteria die when exposed to equivalent concentrations of other acids such as hydrochloric acid. Many bacteria.


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Thiobacillus ferrooxidans and soluble exopolymer production by Avik Nath Download PDF EPUB FB2

The most-studied leaching bacteria are from the genus hiobacillus ferrooxidans and A. thiooxidans are acidophilic mesophiles and together with the moderate thermophile, A. caldus, they belong to the Gram-negative γ-proteobacteria [25].The A. ferrooxidans has been considered as a model biomining microorganism [17, 22] and its cells can be seen by scanning electron.

Under such conditions, soluble Fe 3+ reacts readily with more pyrite and oxidizes it due to the action of acidophilic chemolithotroph bacteria, particularly Acidithiobacillus ferrooxidans []. The dissolution of the most common arsenic sulfide minerals orpiment and arsenopyrite follows the same mechanism and largely depends upon pH.

Bhupinder Dhir, in Transgenic Plant Technology for Remediation of Toxic Metals and Metalloids, Other Approaches for Remediation of Hg. Enzymes such as cytochrome c oxidase from iron (Fe 2+)-oxidizing Hg-sensitive acidophilic bacterium Thiobacillus ferrooxidans also play a role in reduction of Hg 2+ (Iwahori et al., ).Mercury sensitive dissimilatory metal reducing bacteria.

José Manuel Gómez, Domingo Cantero, in Progress in Biotechnology, Immobilisation procedure. Thiobacillus ferrooxidans was immobilised on nickel alloy fibre according to the following procedure: a fixed amount of support was placed in a 1L flask with ml of liquid medium (Fe +2 concentration over mg/L) and 10% (v/v) inoculum.

To adjust the pH to H 2 SO 4 5M was added. D.B. Johnson, in Encyclopedia of Microbiology (Third Edition), Thiobacillus ferrooxidans and soluble exopolymer production book Producers in Acidic Environments. The first extremely acidophilic microorganism to be isolated and characterized was the sulfur-oxidizing bacterium, Acidithiobacillus (At.) thiooxidans (then referred to as Thiobacillus thiooxidans) by Waksman and Joffe in Some years later, another sulfur-oxidizing bacterium was.

Other articles where Thiobacillus is discussed: bacteria: Autotrophic metabolism: Thiobacillus oxidizes thiosulfate and elemental sulfur to sulfate, and A.

ferrooxidans oxidizes ferrous ions to the ferric form. This diverse oxidizing ability allows A. ferrooxidans to tolerate high concentrations of many different ions, including iron, copper, cobalt, nickel, and zinc. Background. Acidithiobacillus ferrooxidans is a Gram-negative, γ-proteobacterium that thrives optimally at 30°C and pH 2, but can grow at pH 1 or lower [].It is abundant in natural environments associated with pyritic ore bodies, coal deposits, and their acidified drainages [2,3].It is an important member of microbial consortia used to recover copper via a process known as bioleaching or.

Thiobacillus ferrooxidans is a gram-negative acidophilic chemolithoautotroph, using CO 2 as a carbon source and obtaining its energy for growth from the oxidation of ferrous iron, sulfur, and reduced sulfur compounds ().

ferrooxidans was initially isolated from acidic copper-leaching waters and believed to be the dominant bacterium responsible for metal sulfide solubilization (). Pronk JT, et al. Energy transduction by anaerobic ferric iron respiration in Thiobacillus ferrooxidans. Appl. Environ. Microbiol.

Irazabal N, et al. Genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC J. Bacteriol.PubMed: Abstract. A rapid and sensitive spectrophotometric procedure was developed for monitoring the growth of Thiobacillus ferrooxidans in liquid culture.

Values determined for the optical densities at nm of washed T. ferrooxidans cell suspensions were directly proportional to both total cell number and total cell protein concentration and provided an accurate measurement of culture growth rate.

It has been demonstrated that Thiobacillus denitrificans may be readily cultivated anaerobically in batch reactors on H 2 S (g) under sulfide‐limiting conditions. Under these conditions sulfide concentrations in the culture medium were less than 1 μM, resulting in very low concentrations of H 2 S in the reactor outlet gas.

The stoichiometry of the reaction was determined, and stable. A. ferrooxidans is a Gram negative rod shaped bacterium that is commonly found in deep caves or acid mine drainage, such as coal waste (10, 11, 12). These acidophilic bacteria thrive in optimal pH level of – where they convert insoluble metals to their soluble state.

Acidithiobacillus ferrooxidans is by far the most widely studied of all extremely acidophilic prokaryotes. While it is found in many types of natural low-pH environments in a variety of geoclimatic contexts, it has been more widely cited in anthropogenic (mostly mine-impacted) environments.

It is responsible for accelerating the oxidative dissolution of sulfide minerals, causing. A method for enumeration of viable numbers of Thiobacillus ferrooxidans using membrane filters on ferrous-iron agar is presented.

Factors affecting colony production were the concentration and brand of agar, pH of the medium, and type of membrane filter. The results suggest that inhibition of T. ferrooxidans by agar is a result of the acid hydrolysis of agar, the main product of which is d.

Acidithiobacillus thiooxidans, formerly known as Thiobacillus thiooxidans until its reclassification into the newly designated genus Acidithiobacillus of the Acidithiobacillia subclass of Proteobacteria, is a Gram-negative, rod-shaped bacterium that uses sulfur as its primary energy source.

It is mesophilic, with a temperature optimum of 28 °C. This bacterium is commonly found in soil, sewer. Iron- and sulfur-oxidizing bacteria identified as Thiobacillus ferrooxidans and T. thiooxidans were successfully enriched from various soil samples contaminated with heavy metals and organic compounds.

Depending on the growth medium, the soil sample, and the type of contaminant, the indigenous isolates solubilized > 50% of most of the heavy metals present in the solid sample (As, Cd.

Abstract. The present article illustrates the increased interest which is manifested in the microorganisms, Thiobacillus ferrooxidans, involved in the biohydrometallurgical extraction wide varieties of problems currently studied are very important in order to gain a better understanding about the factors which are governing the growth of microorganisms, and as a consequence, the.

Thiobacillus thiooxidans and Thiobacillus concretivorus (= T. thiooxidans) grown on elemental sulfur. Formate oxida-tion rates of ferrous iron-grown cells of T. ferrooxidans varied with the strain used (Table 1). The highest rates of formate-dependent oxygen uptake were observed with T.

ferrooxidans. Nucleotide sequence of the gene encoding the nitrogenase iron protein of Thiobacillus ferrooxidans.

J Bacteriol. Jan; (1)– [PMC free article] Ramesar RS, Woods DR, Rawlings DE. Cloning and expression in Escherichia coli of a recA-like gene from the acidophilic autotroph Thiobacillus ferrooxidans. J Gen Microbiol. Di antara kelompok Thiobacilli, Thiobacillus ferrooxidans telah muncul sebagai sebuah bakteri ekonomi yang signifikan di bidang pencucian bijih sulfida sejak penemuannya pada oleh Colmer et al.

Penemuan T. ferrooxidans menyebabkan pengembangan cabang baru dari ilmu metalurgi disebut “biohydrometallurgy” yang berurusan dengan semua. Leaching bacteria such as Thiobacillus ferrooxidans attach to pyrite or sulfur by means of extracellular polymeric substances (EPS) (lipopolysaccharides). The primary attachment to pyrite at pH 2 is mediated by exopolymer-complexed iron(III) ions in an electrochemical interaction with the negatively charged pyrite surface.

EPS from sulfur cells possess increased hydrophobic properties and do.Find out information about Thiobacillus ferrooxidans. An aerobic rod-shaped microorganism that derives its energy from the oxidation of various sulfide minerals and soluble ferrous ion ; it thrives in acidic Explanation of Thiobacillus ferrooxidans.Experimental variables in the manometric study of iron oxidation by washed cell suspensions of the obligate chemoautotroph Thiobacillus ferrooxidans have been examined.

To obtain maximum respiration rates, extremely low cell concentrations (11–15 μg nitrogen) must be used, the substrate level must be between arid μmoles Fe ++ in the form of ferrous sulfate, and physiologically.